Journal:

Article Title: Developmental decoupling of alternative phenotypes: insights from the transcriptomes of horn-polyphenic beetles

doi: 10.1111/j.1558-5646.2010.01106.x

Figure Lengend Snippet: List of Microarray Hybridizations For the majority of microarrays, different tissues were compared within the same morph or sex – for instance, hybridization between head horn epidermis and abdominal epidermis of horned males. For a subset of arrays (N = 4), the same tissues were compared between different morphs. For each microarray (total N = 71), tissue samples originated from four individual beetles. Abbreviations: HM = horned male; SM = sneaker male; F = female; OT = Onthophagus taurus ; ON = Onthophagus nigriventris . All processed and raw microarray data are available at NCBI’s Gene Expression Omnibus http://www.ncbi.nlm.nih.gov/geo/ , series accession number {"type":"entrez-geo","attrs":{"text":"GSE23425","term_id":"23425"}} GSE23425 .

Article Snippet: The cDNA microarrays were printed by the Center for Genomics and Bioinformatics at Indiana University on GAPSII Microarray Slides (Corning) using an Omnigrid 300 Printing Robot and developed protocols ( Andrews et al. 2006 ; Kijimoto et al. 2009 ).

Techniques: Microarray, Hybridization, Gene Expression

Journal: Marine Biotechnology (New York, N.y.)

Article Title: Development and Experimental Validation of a 20K Atlantic Cod ( Gadus morhua ) Oligonucleotide Microarray Based on a Collection of over 150,000 ESTs

doi: 10.1007/s10126-010-9335-6

Figure Lengend Snippet: QPCR results for genes identified as Asal-responsive by microarray and SSH. Average relative quantity ( RQ ) values with SEM error bars. Gene expression differences were determined by t tests on RQ values with a p value cutoff of 0.05. Statistically significant differences between treatments within time points are indicated with an asterisk . Statistically significant differences between time points within treatments are indicated with letters ( lowercase for PBS, uppercase for A. salmonicida ; different letters indicate significant difference). Fold upregulation was calculated as (average RQ 24 HPI)/(average RQ 0 h) for both PBS and Asal groups. Fold downregulation was calculated as 1/(fold upregulation). CAMP, GmSCYA123, HAMP, and IL8 were analyzed previously by QPCR using the same spleen samples but with a different QPCR instrument and using technical duplicates instead of technical triplicates (Feng et al. ). QPCR for these genes was repeated for the current study to ensure that all genes were analyzed using the same instrument and protocol

Article Snippet: Probes were resuspended in sodium phosphate buffer (Schott-Nexterion Spot; Schott, Louisville, KY), to a final concentration of 30 μM, in Genetix X7020 384-well plates (Genetics, Boston, MA) using a Janus liquid-handling robot (PerkinElmer, Waltham, MA) and spotted in duplicate on epoxy microarray slides (Schott-Nexterion Slide E) using an OmniGrid 100 microarrayer (Genomic Solutions, Ann Arbor, MI) equipped with SMT-S50 silicon print pins (Parallel Synthesis Technologies, Santa Clara, CA).

Techniques: Microarray, Expressing

Journal: Marine Biotechnology (New York, N.y.)

Article Title: Development and Experimental Validation of a 20K Atlantic Cod ( Gadus morhua ) Oligonucleotide Microarray Based on a Collection of over 150,000 ESTs

doi: 10.1007/s10126-010-9335-6

Figure Lengend Snippet: QPCR results for genes identified as Asal-responsive by microarray only. Average relative quantity ( RQ ) values with SEM error bars. Gene expression differences were determined by t tests on RQ values with a p value cutoff of 0.05. Statistically significant differences between treatments within time points are indicated with an asterisk . Statistically significant differences between time points within treatments are indicated with letters ( lowercase for PBS, uppercase for A. salmonicida ; different letters indicate significant difference). Fold upregulation was calculated as (average RQ 24 HPI)/(average RQ 0 h) for both PBS and Asal groups. Fold downregulation was calculated as 1/(fold upregulation)

Article Snippet: Probes were resuspended in sodium phosphate buffer (Schott-Nexterion Spot; Schott, Louisville, KY), to a final concentration of 30 μM, in Genetix X7020 384-well plates (Genetics, Boston, MA) using a Janus liquid-handling robot (PerkinElmer, Waltham, MA) and spotted in duplicate on epoxy microarray slides (Schott-Nexterion Slide E) using an OmniGrid 100 microarrayer (Genomic Solutions, Ann Arbor, MI) equipped with SMT-S50 silicon print pins (Parallel Synthesis Technologies, Santa Clara, CA).

Techniques: Microarray, Expressing